Purma Biologics LLC

In advanced disease modeling, the choice of a cell culture medium can play a significant role in providing the correct results. For many researchers, IMDM, or Iscove’s Modified Dulbecco's Medium , is preferred. What reasons may lead to the choice of IMDM over the others? What is IMDM Media? IMDM is a highly enriched cell culture medium that provides all necessary cell growth and maintenance factors, including essential nutrients, amino acids, and vitamins. It was particularly formulated to support a wide range of cell types, including those desirable for elaborate disease models. Why Choose IMDM Medium for Disease Modeling? The choice of the medium is, in disease modeling, a significant factor in determining how accurate your results are. IMDM medium has a good nutritive profile that helps reproduce in vivo conditions. This is the reason why this medium is useful in the modeling of diseases like cancers or even neurodegenerative disorders that require highly specific cond...

PBS, or  phosphate-buffered saline , is an essential element in the formulation and development of vaccines. PBS is a very simple yet versatile buffer solution applied in the laboratory, and it provides stability, isotonicity, and compatibility needed for the preservation of biological samples and research associated with vaccination. This blog covers the essential roles of PBS in vaccine research, its preparation, and its application to vaccines for efficacy and safety. What Exactly Is Phosphate-Buffered Saline? Phosphate-buffered saline is basically an aqueous solution of salts consisting of sodium chloride and sodium phosphate, and in some formulations, potassium chloride and potassium phosphate. Due to its stability of pH and isotonicity, it has found extensive application in biological and biochemical research, similar to that of the human body. It is also easy and consistent to prepare phosphate-buffered saline, thereby making it a first-rate component found in laboratory env...

F 12 Medium is an emerging requirement that has become vital in regenerative medicine applications, including cell culture, tissue engineering, and therapeutic developments. It was actually designed for the growth of a wide variety of cell types, but it is increasingly regarded for regenerative applications in the fields where it is being put to use. Here are the various applications of F12 media in regenerative medicine. Stem Cell Culture and Maintenance One of the major uses of the F12 medium is for maintaining and expanding stem cell populations. F-12 is a favorable medium for normal growth of most types of stem cells, including mesenchymal stem cells (MSC), hematopoietic stem cells (HSC), and induced pluripotent stem cells (iPSC). It forms the basis for regenerative therapy because these cells have the potential to regenerate all kinds of cells for the repair or replacement of damaged tissues.   Tissue Engineering and Regeneration   F- 12 medium serves as a grow...

  In cell culture, the appropriateness of a chosen buffer system becomes critical with respect to how physiological conditions are maintained, enabling successful runs of experiments. The appropriate pH and ionic balance are among crucial factors with respect to the cell's survivability; thus, this role lies in buffers, of which PBS (phosphate buffered saline) buffer is quite the most prevalent one for use. But how is PBS buffer for cell culture different from others, and what should be considered before the right choice is picked? What is PBS? The buffer composed of sodium chloride, potassium chloride, and phosphate salts makes PBS an isotonic solution. PBS is mainly applied in cell culture to allow the maintenance of a pH of approximately 7.4 which is close to physiologic conditions. Although mostly buffering pH, PBS holds the cells hydrated and relatively stable by maintaining osmotic pressure. Its composition avoids disturbances of cell membranes at best, which makes the s...

 With advances in cell culture techniques, scientists are always on a quest to find newer and more innovative formulations of media that tend to emphasize more on cell growth, viability, and output of experiments. More importantly, due to inconsistency of Fetal Bovine Serum (FBS), there is a developing demand toward those media which need less or no FBS. Advanced DMEM F12 is among the versatile and robust choices for this purpose in cell biology applications. Below, we shall discuss the future trend in the media of cell culture and how the  DMEM F12 formulation  fits into that. Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) 1. Reduced serum use focus The most pronounced trend has been toward serum-free or low-serum media formulations. Researchers more and more remark that both the heterogeneity and the ethical concerns over Fetal Bovine Serum (FBS) give scientific justification for serum-free formulations as necessary for  Advanced DMEM F12 , but again, ideally,...

The antibodies in human body fabricates to defend itself are proteins; They can also be produced in labs that function just as natural ones produced by mAbs (monoclonal bodies) or mAbs (monoclonal Abs). When mAbs are generated in labs, are done by fusing the cancer cells with spleen cells of mouse, so when these cells divide and produce monoclonal antibodies. The mAb generating cells can be cryopreserved in liquid nitrogen until that mAb is needed again. Some of the mAbs are antibodies, your immune system component. They roam around in our bodies to find the antigens (foreign elements) they are supposed to kill. Science: Lab-made monoclonals boost your immunity. The term “monoclonal” describes the nature of laboratory-produced antibodies as clones. They are one antibody’s exact duplicates. The letters “mab” are frequently added to the end of the product names in their generic forms. Challenges During Producing Monoclonal Antibodies Monoclonal antibodies  are produced based on hybri...