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PBS Buffer Vs. Other Buffers: How To Select The Right One For Cell Culture

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  In cell culture, the appropriateness of a chosen buffer system becomes critical with respect to how physiological conditions are maintained, enabling successful runs of experiments. The appropriate pH and ionic balance are among crucial factors with respect to the cell's survivability; thus, this role lies in buffers, of which PBS (phosphate buffered saline) buffer is quite the most prevalent one for use. But how is PBS buffer for cell culture different from others, and what should be considered before the right choice is picked? What is PBS? The buffer composed of sodium chloride, potassium chloride, and phosphate salts makes PBS an isotonic solution. PBS is mainly applied in cell culture to allow the maintenance of a pH of approximately 7.4 which is close to physiologic conditions. Although mostly buffering pH, PBS holds the cells hydrated and relatively stable by maintaining osmotic pressure. Its composition avoids disturbances of cell membranes at best, which makes the s

Future Trends in Cell Culture Media: The Role of DMEM F12 Advanced

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 With advances in cell culture techniques, scientists are always on a quest to find newer and more innovative formulations of media that tend to emphasize more on cell growth, viability, and output of experiments. More importantly, due to inconsistency of Fetal Bovine Serum (FBS), there is a developing demand toward those media which need less or no FBS. Advanced DMEM F12 is among the versatile and robust choices for this purpose in cell biology applications. Below, we shall discuss the future trend in the media of cell culture and how the  DMEM F12 formulation  fits into that. Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) 1. Reduced serum use focus The most pronounced trend has been toward serum-free or low-serum media formulations. Researchers more and more remark that both the heterogeneity and the ethical concerns over Fetal Bovine Serum (FBS) give scientific justification for serum-free formulations as necessary for  Advanced DMEM F12 , but again, ideally, the medium should

How Monoclonal Antibodies (mAb) Are Transforming Healthcare

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The antibodies in human body fabricates to defend itself are proteins; They can also be produced in labs that function just as natural ones produced by mAbs (monoclonal bodies) or mAbs (monoclonal Abs). When mAbs are generated in labs, are done by fusing the cancer cells with spleen cells of mouse, so when these cells divide and produce monoclonal antibodies. The mAb generating cells can be cryopreserved in liquid nitrogen until that mAb is needed again. Some of the mAbs are antibodies, your immune system component. They roam around in our bodies to find the antigens (foreign elements) they are supposed to kill. Science: Lab-made monoclonals boost your immunity. The term “monoclonal” describes the nature of laboratory-produced antibodies as clones. They are one antibody’s exact duplicates. The letters “mab” are frequently added to the end of the product names in their generic forms. Challenges During Producing Monoclonal Antibodies Monoclonal antibodies  are produced based on hybridoma