PBS Buffer Vs. Other Buffers: How To Select The Right One For Cell Culture
In cell culture, the appropriateness of a chosen buffer system becomes critical with respect to how physiological conditions are maintained, enabling successful runs of experiments. The appropriate pH and ionic balance are among crucial factors with respect to the cell's survivability; thus, this role lies in buffers, of which PBS (phosphate buffered saline) buffer is quite the most prevalent one for use. But how is PBS buffer for cell culture different from others, and what should be considered before the right choice is picked? What is PBS? The buffer composed of sodium chloride, potassium chloride, and phosphate salts makes PBS an isotonic solution. PBS is mainly applied in cell culture to allow the maintenance of a pH of approximately 7.4 which is close to physiologic conditions. Although mostly buffering pH, PBS holds the cells hydrated and relatively stable by maintaining osmotic pressure. Its composition avoids disturbances of cell membranes at best, which makes the s